|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse MCAM ORF mammalian expression plasmid, C-GFPSpark tag||MG50794-ACG|
|Mouse MCAM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50794-ACR|
|Mouse MCAM ORF mammalian expression plasmid, C-Flag tag||MG50794-CF|
|Mouse MCAM ORF mammalian expression plasmid, C-His tag||MG50794-CH|
|Mouse MCAM ORF mammalian expression plasmid, C-Myc tag||MG50794-CM|
|Mouse MCAM ORF mammalian expression plasmid, C-HA tag||MG50794-CY|
|Mouse MCAM Gene cDNA clone plasmid||MG50794-G|
|Mouse MCAM ORF mammalian expression plasmid, N-Flag tag||MG50794-NF|
|Mouse MCAM ORF mammalian expression plasmid, N-His tag||MG50794-NH|
|Mouse MCAM ORF mammalian expression plasmid, N-Myc tag||MG50794-NM|
|Mouse MCAM ORF mammalian expression plasmid, N-HA tag||MG50794-NY|
|Mouse MCAM natural ORF mammalian expression plasmid||MG50794-UT|
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The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.