|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse SIGLEC5 ORF mammalian expression plasmid, C-GFPSpark tag||MG50836-ACG|
|Mouse SIGLEC5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50836-ACR|
|Mouse SIGLEC5 ORF mammalian expression plasmid, C-Flag tag||MG50836-CF|
|Mouse SIGLEC5 ORF mammalian expression plasmid, C-His tag||MG50836-CH|
|Mouse SIGLEC5 ORF mammalian expression plasmid, C-Myc tag||MG50836-CM|
|Mouse SIGLEC5 ORF mammalian expression plasmid, C-HA tag||MG50836-CY|
|Mouse SIGLEC5 Gene cDNA clone plasmid||MG50836-G|
|Mouse SIGLEC5 ORF mammalian expression plasmid, N-Flag tag||MG50836-NF|
|Mouse SIGLEC5 ORF mammalian expression plasmid, N-His tag||MG50836-NH|
|Mouse SIGLEC5 ORF mammalian expression plasmid, N-Myc tag||MG50836-NM|
|Mouse SIGLEC5 ORF mammalian expression plasmid, N-HA tag||MG50836-NY|
|Mouse SIGLEC5 natural ORF mammalian expression plasmid||MG50836-UT|
|Learn more about expression Vectors|
SIGLEC5 contains 2 Ig-like C2-type (immunoglobulin-like) domains and 1 Ig-like V-type (immunoglobulin-like) domain. It belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. SIGLEC5 is expressed by monocytic/myeloid lineage cells. It is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. It is also expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation. SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.