|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse THOP1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50828-ACG|
|Mouse THOP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50828-ACR|
|Mouse THOP1 ORF mammalian expression plasmid, N-GFPSpark tag||MG50828-ANG|
|Mouse THOP1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50828-ANR|
|Mouse THOP1 ORF mammalian expression plasmid, C-Flag tag||MG50828-CF|
|Mouse THOP1 ORF mammalian expression plasmid, C-His tag||MG50828-CH|
|Mouse THOP1 ORF mammalian expression plasmid, C-Myc tag||MG50828-CM|
|Mouse THOP1 ORF mammalian expression plasmid, C-HA tag||MG50828-CY|
|Mouse THOP1 Gene cDNA clone plasmid||MG50828-G|
|Mouse THOP1 ORF mammalian expression plasmid, N-Flag tag||MG50828-NF|
|Mouse THOP1 ORF mammalian expression plasmid, N-His tag||MG50828-NH|
|Mouse THOP1 ORF mammalian expression plasmid, N-Myc tag||MG50828-NM|
|Mouse THOP1 ORF mammalian expression plasmid, N-HA tag||MG50828-NY|
|Mouse THOP1 natural ORF mammalian expression plasmid||MG50828-UT|
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THOP1, also known as Thimet oligopeptidase 1, Thimet oligopeptidase, EC 22.214.171.124, or EP24.15, is a zinc(II) endopeptidase implicated in the processing of numerous physiological peptides. As an intracellular enzyme, highly expressed in the brain, kidneys and neuroendocrine tissue, THOP1 has been proposed to metabolize peptides within cells, thereby affecting antigen presentation and G protein-coupled receptor signal transduction. Its substrates is gonadotrophin-releasing hormone (GnRH), an important hypothalamic hormone that regulates the synthesis and release of oestradiol and facilitates female sexual behaviour. THOP1 against toxic effects of Abeta in the early stages of Alzheimer disease (AD) pathology, and suggest that the observed increase in THOP1 expression might be part of a compensatory defense mechanism of the brain against an increased Abeta load.