|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat ADK ORF mammalian expression plasmid, C-GFPSpark tag||RG80662-ACG|
|Rat ADK ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80662-ACR|
|Rat ADK ORF mammalian expression plasmid, N-GFPSpark tag||RG80662-ANG|
|Rat ADK ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80662-ANR|
|Rat ADK ORF mammalian expression plasmid, C-Flag tag||RG80662-CF|
|Rat ADK ORF mammalian expression plasmid, C-His tag||RG80662-CH|
|Rat ADK ORF mammalian expression plasmid, C-Myc tag||RG80662-CM|
|Rat ADK ORF mammalian expression plasmid, C-HA tag||RG80662-CY|
|Rat ADK ORF mammalian expression plasmid, N-Flag tag||RG80662-NF|
|Rat ADK ORF mammalian expression plasmid, N-His tag||RG80662-NH|
|Rat ADK ORF mammalian expression plasmid, N-Myc tag||RG80662-NM|
|Rat ADK ORF mammalian expression plasmid, N-HA tag||RG80662-NY|
|Rat ADK Gene cDNA clone plasmid||RG80662-U|
|Rat ADK natural ORF mammalian expression plasmid||RG80662-UT|
|Learn more about expression Vectors|
Adenosine kinase(ADK) belongs to the family of transferases. Adenosine kinase (ADK) is the key enzyme in adenosine metabolism and catalyzes ATP and adenosine into two products: ADP and AMP. Two isoforms of the enzyme adenosine kinase (ADK), which differ at their N-terminal ends, are found in mammalian cells. It has been shown that the two ADK isoforms differ only in their first exons and the promoter regions; hence they arise via differential splicing of their first exons with the other exons common to both isoforms. In adult brain, ADK is primarily present in astrocytes. Several lines of experimental evidence support a critical role of ADK in different types of brain injury associated with astrogliosis, which is also a prominent morphologic feature of temporal lobe epilepsy (TLE). It has been suggested that dysregulation of ADK in astrocytes is a common pathologic hallmark of TLE. Moreover, in vitro data suggest the existence of an additional layer of modulatory crosstalk between the astrocyte-based adenosine cycle and inflammation. ADK also contributes to CK homeostasis in vivo.