|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat DCN ORF mammalian expression plasmid, C-GFPSpark tag||RG80656-ACG|
|Rat DCN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80656-ACR|
|Rat DCN ORF mammalian expression plasmid, C-Flag tag||RG80656-CF|
|Rat DCN ORF mammalian expression plasmid, C-His tag||RG80656-CH|
|Rat DCN ORF mammalian expression plasmid, C-Myc tag||RG80656-CM|
|Rat DCN ORF mammalian expression plasmid, C-HA tag||RG80656-CY|
|Rat DCN ORF mammalian expression plasmid, N-Flag tag||RG80656-NF|
|Rat DCN ORF mammalian expression plasmid, N-His tag||RG80656-NH|
|Rat DCN ORF mammalian expression plasmid, N-Myc tag||RG80656-NM|
|Rat DCN ORF mammalian expression plasmid, N-HA tag||RG80656-NY|
|Rat DCN Gene cDNA clone plasmid||RG80656-U|
|Rat DCN natural ORF mammalian expression plasmid||RG80656-UT|
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Decorin is a ubiquitous small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a core protein and a covalently linked glycosaminoglycan chain which is either chondroitin sulfate (CS) or dermatan sulfate (DS). As a component of connective tissue, decorin interacts with several extracellular matrix components, such as type I collagen and fibronectin, and plays a role in matrix assembly. Decorin resides in the tumor microenvironment and affects the biology of various types of cancer by downregulating the activity of several receptors involved in cell growth and survival. Decorin binds to and modulates the signaling of the epidermal growth factor receptor and other members of the ErbB family of receptor tyrosine kinases. It exerts its antitumor activity by a dual mechanism: via inhibition of these key receptors through their physical downregulation coupled with attenuation of their signaling, and by binding to and sequestering TGFbeta. Decorin also modulates the insulin-like growth factor receptor and the low-density lipoprotein receptor-related protein 1, which indirectly affects the TGFbeta receptor pathway. Decorin plays significant roles in tissue development and assembly, as well as playing both direct and indirect signaling roles.