|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CAR10 ORF mammalian expression plasmid, C-GFPSpark tag||MG50768-ACG|
|Mouse CAR10 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50768-ACR|
|Mouse CAR10 ORF mammalian expression plasmid, N-GFPSpark tag||MG50768-ANG|
|Mouse CAR10 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50768-ANR|
|Mouse CAR10 ORF mammalian expression plasmid, C-Flag tag||MG50768-CF|
|Mouse CAR10 ORF mammalian expression plasmid, C-His tag||MG50768-CH|
|Mouse CAR10 ORF mammalian expression plasmid, C-Myc tag||MG50768-CM|
|Mouse CAR10 ORF mammalian expression plasmid, C-HA tag||MG50768-CY|
|Mouse CAR10 Gene cDNA clone plasmid||MG50768-G|
|Mouse CAR10 ORF mammalian expression plasmid, N-Flag tag||MG50768-NF|
|Mouse CAR10 ORF mammalian expression plasmid, N-His tag||MG50768-NH|
|Mouse CAR10 ORF mammalian expression plasmid, N-Myc tag||MG50768-NM|
|Mouse CAR10 ORF mammalian expression plasmid, N-HA tag||MG50768-NY|
|Mouse CAR10 natural ORF mammalian expression plasmid||MG50768-UT|
|Learn more about expression Vectors|
Carbonic anhydrase X, also called carbonic anhydrase - related protein X (CARPX) and CA10, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene.