|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse BGN ORF mammalian expression plasmid, C-GFPSpark tag||MG50766-ACG|
|Mouse BGN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50766-ACR|
|Mouse BGN ORF mammalian expression plasmid, C-Flag tag||MG50766-CF|
|Mouse BGN ORF mammalian expression plasmid, C-His tag||MG50766-CH|
|Mouse BGN ORF mammalian expression plasmid, C-Myc tag||MG50766-CM|
|Mouse BGN ORF mammalian expression plasmid, C-HA tag||MG50766-CY|
|Mouse BGN Gene cDNA clone plasmid||MG50766-G|
|Mouse BGN ORF mammalian expression plasmid, N-Flag tag||MG50766-NF|
|Mouse BGN ORF mammalian expression plasmid, N-His tag||MG50766-NH|
|Mouse BGN ORF mammalian expression plasmid, N-Myc tag||MG50766-NM|
|Mouse BGN ORF mammalian expression plasmid, N-HA tag||MG50766-NY|
|Mouse BGN natural ORF mammalian expression plasmid||MG50766-UT|
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Biglycan, also known as PG-S1 and BGN, is a a small leucine-rich repeat proteoglycan (SLRP). It can be detected in a variety of extracellular matrix tissues, including bone, cartilage and tendon. Biglycan consists of a protein core containing leucine-rich repeat regions and two glycosaminoglycan (GAG) chains consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS). Non-glycanated forms of biglycan (no GAG chains) increase with age in human articular cartilage. Biglycan interacts with collagen, both via the core protein and GAG chains. Biglycan plays a role in the mineralisation of bone. Biglycan core protein binds to the growth factors BMP-4 and influences its bioactivity.