|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse TNFRSF4 ORF mammalian expression plasmid, C-GFPSpark tag||MG50808-ACG|
|Mouse TNFRSF4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50808-ACR|
|Mouse TNFRSF4 ORF mammalian expression plasmid, C-Flag tag||MG50808-CF|
|Mouse TNFRSF4 ORF mammalian expression plasmid, C-His tag||MG50808-CH|
|Mouse TNFRSF4 ORF mammalian expression plasmid, C-Myc tag||MG50808-CM|
|Mouse TNFRSF4 ORF mammalian expression plasmid, C-HA tag||MG50808-CY|
|Mouse TNFRSF4 Gene cDNA clone plasmid||MG50808-G|
|Mouse TNFRSF4 ORF mammalian expression plasmid, N-Flag tag||MG50808-NF|
|Mouse TNFRSF4 ORF mammalian expression plasmid, N-His tag||MG50808-NH|
|Mouse TNFRSF4 ORF mammalian expression plasmid, N-Myc tag||MG50808-NM|
|Mouse TNFRSF4 ORF mammalian expression plasmid, N-HA tag||MG50808-NY|
|Mouse TNFRSF4 natural ORF mammalian expression plasmid||MG50808-UT|
|Learn more about expression Vectors|
OX40 (CD134) and its binding partner, OX40L (CD252), are members of the tumor necrosis factor receptor/tumor necrosis factor superfamily, is known to break an existing state of tolerance in malignancies, leading to a reactivation of antitumor immunity. The interaction between OX40 and OX40L plays an important role in antigen-specific T-cell expansion and survival. OX40 and OX40L also regulate cytokine production from T cells, antigen-presenting cells, natural killer cells, and natural killer T cells, and modulate cytokine receptor signaling. In line with these important modulatory functions, OX40-OX40L interactions have been found to play a central role in the development of multiple inflammatory and autoimmune diseases, making them attractive candidates for intervention in the clinic. Conversely, stimulating OX40 has shown it to be a candidate for therapeutic immunization strategies for cancer and infectious disease.