|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse LILRA5 ORF mammalian expression plasmid, C-GFPSpark tag||MG50762-ACG|
|Mouse LILRA5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50762-ACR|
|Mouse LILRA5 ORF mammalian expression plasmid, C-Flag tag||MG50762-CF|
|Mouse LILRA5 ORF mammalian expression plasmid, C-His tag||MG50762-CH|
|Mouse LILRA5 ORF mammalian expression plasmid, C-Myc tag||MG50762-CM|
|Mouse LILRA5 ORF mammalian expression plasmid, C-HA tag||MG50762-CY|
|Mouse LILRA5 Gene cDNA clone plasmid||MG50762-G|
|Mouse LILRA5 ORF mammalian expression plasmid, N-Flag tag||MG50762-NF|
|Mouse LILRA5 ORF mammalian expression plasmid, N-His tag||MG50762-NH|
|Mouse LILRA5 ORF mammalian expression plasmid, N-Myc tag||MG50762-NM|
|Mouse LILRA5 ORF mammalian expression plasmid, N-HA tag||MG50762-NY|
|Mouse LILRA5 natural ORF mammalian expression plasmid||MG50762-UT|
|Learn more about expression Vectors|
LILRA5 is a member of the leukocyte immunoglobulin-like receptor (LIR) family. LILR are a family of receptors possessing extracellular immunoglobulin domains. They are also known as CD85, ILTs and LIR, and can exert immunomodulatory effects on a wide range of immune cells. ILT-11 contains 2 Ig-like C2-type (immunoglobulin-like) domains. It can be detected n tissues of the hematopoietic system, including bone marrow, spleen, lymph node and peripheral leukocytes. Crosslink of ILT-11 on the surface of monocytes has been shown to induce calcium flux and secretion of several proinflammatory cytokines, which suggests the roles of this protein in triggering innate immune responses.