|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse PDCD1LG2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50804-ACG|
|Mouse PDCD1LG2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50804-ACR|
|Mouse PDCD1LG2 ORF mammalian expression plasmid, C-Flag tag||MG50804-CF|
|Mouse PDCD1LG2 ORF mammalian expression plasmid, C-His tag||MG50804-CH|
|Mouse PDCD1LG2 ORF mammalian expression plasmid, C-Myc tag||MG50804-CM|
|Mouse PDCD1LG2 ORF mammalian expression plasmid, C-HA tag||MG50804-CY|
|Mouse PDCD1LG2 Gene cDNA clone plasmid||MG50804-G|
|Mouse PDCD1LG2 ORF mammalian expression plasmid, N-Flag tag||MG50804-NF|
|Mouse PDCD1LG2 ORF mammalian expression plasmid, N-His tag||MG50804-NH|
|Mouse PDCD1LG2 ORF mammalian expression plasmid, N-Myc tag||MG50804-NM|
|Mouse PDCD1LG2 ORF mammalian expression plasmid, N-HA tag||MG50804-NY|
|Mouse PDCD1LG2 natural ORF mammalian expression plasmid||MG50804-UT|
|Learn more about expression Vectors|
Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.