|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse USH1C ORF mammalian expression plasmid, C-GFPSpark tag||MG50803-ACG|
|Mouse USH1C ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50803-ACR|
|Mouse USH1C ORF mammalian expression plasmid, N-GFPSpark tag||MG50803-ANG|
|Mouse USH1C ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50803-ANR|
|Mouse USH1C ORF mammalian expression plasmid, C-Flag tag||MG50803-CF|
|Mouse USH1C ORF mammalian expression plasmid, C-His tag||MG50803-CH|
|Mouse USH1C ORF mammalian expression plasmid, C-Myc tag||MG50803-CM|
|Mouse USH1C ORF mammalian expression plasmid, C-HA tag||MG50803-CY|
|Mouse USH1C Gene cDNA clone plasmid||MG50803-G|
|Mouse USH1C ORF mammalian expression plasmid, N-Flag tag||MG50803-NF|
|Mouse USH1C ORF mammalian expression plasmid, N-His tag||MG50803-NH|
|Mouse USH1C ORF mammalian expression plasmid, N-Myc tag||MG50803-NM|
|Mouse USH1C ORF mammalian expression plasmid, N-HA tag||MG50803-NY|
|Mouse USH1C natural ORF mammalian expression plasmid||MG50803-UT|
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Harmonin, also known as Antigen NY-CO-38 / NY-CO-37, Autoimmune enteropathy-related antigen AIE-75, Protein PDZ-73, Renal carcinoma antigen NY-REN-3, Usher syndrome type-1C protein and USH1C, is a protein which is expressed in small intestine, colon, kidney, eye and weakly in pancreas. USH1C is expressed also in vestibule of the inner ear. USH1C contains 3 PDZ (DHR) domains. USH1C may be involved in protein-protein interaction. Defects in USH1C are the cause of Usher syndrome type 1C (USH1C), also known as Usher syndrome type I Acadian variety. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa and sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). Defects in USH1C are also the cause of deafness autosomal recessive type 18 (DFNB18) which is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.