|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat PNLIPRP1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80624-ACG|
|Rat PNLIPRP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80624-ACR|
|Rat PNLIPRP1 ORF mammalian expression plasmid, C-Flag tag||RG80624-CF|
|Rat PNLIPRP1 ORF mammalian expression plasmid, C-His tag||RG80624-CH|
|Rat PNLIPRP1 ORF mammalian expression plasmid, C-Myc tag||RG80624-CM|
|Rat PNLIPRP1 ORF mammalian expression plasmid, C-HA tag||RG80624-CY|
|Rat PNLIPRP1 ORF mammalian expression plasmid, N-Flag tag||RG80624-NF|
|Rat PNLIPRP1 ORF mammalian expression plasmid, N-His tag||RG80624-NH|
|Rat PNLIPRP1 ORF mammalian expression plasmid, N-Myc tag||RG80624-NM|
|Rat PNLIPRP1 ORF mammalian expression plasmid, N-HA tag||RG80624-NY|
|Rat PNLIPRP1 Gene cDNA clone plasmid||RG80624-U|
|Rat PNLIPRP1 natural ORF mammalian expression plasmid||RG80624-UT|
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PNLIPRP1, also known as PLRP1, belongs to the AB hydrolase superfamily, Lipase family. PNLIPRP1 is structurally similar to PLRP2. However, these two proteins display different functional properties. PNLIPRP1 may function as inhibitor of dietary triglyceride digestion. It lacks detectable lipase activity towards triglycerides, diglycerides, phosphatidylcholine, galactolipids or cholesterol esters. PLRP2 hydrolyses milk fat with a lower catalytic efficiency than that of PL. PLRP2 activity, higher on homogenized than on native milk fat, is differently influenced by fatty acids and colipase depending on a proteolytic cleavage in the lid domain.