|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CDH2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50752-ACG|
|Mouse CDH2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50752-ACR|
|Mouse CDH2 ORF mammalian expression plasmid, C-Flag tag||MG50752-CF|
|Mouse CDH2 ORF mammalian expression plasmid, C-His tag||MG50752-CH|
|Mouse CDH2 ORF mammalian expression plasmid, C-Myc tag||MG50752-CM|
|Mouse CDH2 ORF mammalian expression plasmid, C-HA tag||MG50752-CY|
|Mouse CDH2 Gene cDNA clone plasmid||MG50752-G|
|Mouse CDH2 ORF mammalian expression plasmid, N-Flag tag||MG50752-NF|
|Mouse CDH2 ORF mammalian expression plasmid, N-His tag||MG50752-NH|
|Mouse CDH2 ORF mammalian expression plasmid, N-Myc tag||MG50752-NM|
|Mouse CDH2 ORF mammalian expression plasmid, N-HA tag||MG50752-NY|
|Mouse CDH2 natural ORF mammalian expression plasmid||MG50752-UT|
|Learn more about expression Vectors|
Cadherins are calcium dependent cell adhesion proteins, and they preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin 2 (CDH2), also known as N-Cadherin (neuronal) (NCAD), is a single-pass tranmembrane protein and a cadherin containing 5 cadherin domains. N-Cadherin displays a ubiquitous expression pattern but with different expression levels between endocrine cell types. CDH2 (NCAD) has been shown to play an essential role in normal neuronal development, which is implicated in an array of processes including neuronal differentiation and migration, and axon growth and fasciculation. In addition, N-Cadherin expression was upregulated in human HSC during activation in culture, and function or expression blocking of N-Cadherin promoted apoptosis. During apoptosis, N-Cadherin was cleaved into 20-100 kDa fragments. It may provide a novel target for therapies that are directed toward intimal proliferative disorders, including restenosis and vascular bypass graft failure. N-Cadherin is associated with tumor aggressiveness and metastatic potential and may contribute to tumor progression.