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Human HDAC3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-tagged

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HDAC3cDNA Clone Product Information
Gene_bank_ref_id:NM_003883.3
cDNA Size:1287
cDNA Description:ORF Clone of Homo sapiens histone deacetylase 3 DNA.
Gene Synonym:HD3, RPD3, RPD3-2, HDAC3
Species:Human
Vector:pCMV3-N-HA
Restriction Site:
Tag Sequence:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Sequence Description:
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pCMV3-N-HA Vector Information
 
Vector Name pCMV3-N-HA
Vector Size 6101bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive, Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag HA
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

pCMV3-N-HA Physical Map
Schematic of pCMV3-N-HA Multiple Cloning Sites

HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

Human HDAC3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-tagged on other vectors
Human HDAC3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG11511-ACG$325
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Human HDAC3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-GFPSpark-taggedHG11511-ANG$325
Human HDAC3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagHG11511-ANR$325
Human HDAC3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG11511-CF$295
Human HDAC3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG11511-CH$295
Human HDAC3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG11511-CM$295
Human HDAC3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG11511-CY$295
Human HDAC3 Gene cDNA Clone (full-length ORF Clone)HG11511-M$95
Human HDAC3 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-taggedHG11511-M-F$295
Human HDAC3 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG11511-M-N$295
Human HDAC3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG11511-NF$295
Human HDAC3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG11511-NH$295
Human HDAC3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG11511-NM$295
Human HDAC3 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG11511-NY$295
Human HDAC3 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG11511-UT$295
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Background

Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general, they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs; classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions (for example HDAC-14.3.3 complexes are retained in the cytosol) and by the class to which they belong (class 1 HDACs are predominantly nuclear whilst class 2 HDACs shuttle between the nucleus and cytosol). HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents. Histone Deacetylases (HDACs) is Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.

References
  • Emiliani S, et al. (1998) Characterization of a human RPD3 ortholog, HDAC3. Proc Natl Acad Sci. 95 (6): 2795-800.
  • Dangond F, et al. (1998) Differential display cloning of a novel human histone deacetylase (HDAC3) cDNA from PHA-activated immune cells. Biochem Biophys Res Commun. 242 (3): 648-52.
  • Nicolas E, et al. (2001) The histone deacetylase HDAC3 targets RbAp48 to the retinoblastoma protein. Nucleic Acids Res. 29 (15): 3131-6.
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    Catalog:HG11511-NY
    List Price: $295.00  (Save $0.00)
    Price:$295.00      [How to order]
    Availability2-3 weeks
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