|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse ABHD4 ORF mammalian expression plasmid, C-GFPSpark tag||MG50747-ACG|
|Mouse ABHD4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50747-ACR|
|Mouse ABHD4 ORF mammalian expression plasmid, N-GFPSpark tag||MG50747-ANG|
|Mouse ABHD4 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50747-ANR|
|Mouse ABHD4 ORF mammalian expression plasmid, C-Flag tag||MG50747-CF|
|Mouse ABHD4 ORF mammalian expression plasmid, C-His tag||MG50747-CH|
|Mouse ABHD4 ORF mammalian expression plasmid, C-Myc tag||MG50747-CM|
|Mouse ABHD4 ORF mammalian expression plasmid, C-HA tag||MG50747-CY|
|Mouse ABHD4 Gene cDNA clone plasmid||MG50747-G|
|Mouse ABHD4 ORF mammalian expression plasmid, N-Flag tag||MG50747-NF|
|Mouse ABHD4 ORF mammalian expression plasmid, N-His tag||MG50747-NH|
|Mouse ABHD4 ORF mammalian expression plasmid, N-Myc tag||MG50747-NM|
|Mouse ABHD4 ORF mammalian expression plasmid, N-HA tag||MG50747-NY|
|Mouse ABHD4 natural ORF mammalian expression plasmid||MG50747-UT|
|Learn more about expression Vectors|
Abhydrolase domain containing 4 (ABHD4), also known as alpha/beta-hydrolase 4 (ABH4) , or lyso-N-acylphosphatidylethanolamine lipase, which belongs to the ABHD4/ABHD5 subfamily of peptidase S33 family. Abhydrolase domain containing (ABHD) gene was a small group belongs to alpha/beta hydrolase superfamily. Known members of this group are all found to be involved in important biochemical processes and related to various diseases. The alpha/beta-hydrolase 4 (ABH4) is a lysophospholipase/phospholipase B that selectively hydrolyzes N-acyl phosphatidylethanolamines (NAPEs) and lysoNAPEs. ABH4 accepts lysoNAPEs bearing both saturated and polyunsaturated N-acyl chains as substrates and displays a distribution that closely mirrors lysoNAPE-lipase activity in mouse tissues. The existence of an NAPE-PLD-independent route for NAE biosynthesis and suggest that ABH4 plays a role in this metabolic pathway by acting as a (lyso)NAPE-selective lipase.