|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse PRKCI ORF mammalian expression plasmid, C-GFPSpark tag||MG50788-ACG|
|Mouse PRKCI ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50788-ACR|
|Mouse PRKCI ORF mammalian expression plasmid, N-GFPSpark tag||MG50788-ANG|
|Mouse PRKCI ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50788-ANR|
|Mouse PRKCI ORF mammalian expression plasmid, C-Flag tag||MG50788-CF|
|Mouse PRKCI ORF mammalian expression plasmid, C-His tag||MG50788-CH|
|Mouse PRKCI ORF mammalian expression plasmid, C-Myc tag||MG50788-CM|
|Mouse PRKCI ORF mammalian expression plasmid, C-HA tag||MG50788-CY|
|Mouse PRKCI Gene cDNA clone plasmid||MG50788-G|
|Mouse PRKCI ORF mammalian expression plasmid, N-Flag tag||MG50788-NF|
|Mouse PRKCI ORF mammalian expression plasmid, N-His tag||MG50788-NH|
|Mouse PRKCI ORF mammalian expression plasmid, N-Myc tag||MG50788-NM|
|Mouse PRKCI ORF mammalian expression plasmid, N-HA tag||MG50788-NY|
|Mouse PRKCI natural ORF mammalian expression plasmid||MG50788-UT|
|Learn more about expression Vectors|
Protein kinase C iota type, also known as Atypical protein kinase C-lambda/iota, aPKC-lambda/iota and PRKCI, is a cytoplasm, membrane and nucleus protein which belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and PKC subfamily. PRKCI contains one AGC-kinase C-terminal domain, one OPR domain, one phorbol-ester/DAG-type zinc finger and one protein kinase domain. PRKCI is predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. It is highly expressed in non-small cell lung cancers. PRKCI is a calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. It may play a role in the secretory response to nutrients. PRKCI is involved in cell polarization processes and the formation of epithelial tight junctions. It is implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. PRKCI functions in both pro- and anti-apoptotic pathways. It functions in the RAC1/ERK signaling required for transformed growth. PRKCI plays a role in microtubule dynamics through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). PRKCI might be a target for novel lipid activators that are elevated during nutrient-stimulated insulin secretion.