|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse VCL ORF mammalian expression plasmid, C-GFPSpark tag||MG50773-ACG|
|Mouse VCL ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50773-ACR|
|Mouse VCL ORF mammalian expression plasmid, N-GFPSpark tag||MG50773-ANG|
|Mouse VCL ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50773-ANR|
|Mouse VCL ORF mammalian expression plasmid, C-Flag tag||MG50773-CF|
|Mouse VCL ORF mammalian expression plasmid, C-His tag||MG50773-CH|
|Mouse VCL ORF mammalian expression plasmid, C-Myc tag||MG50773-CM|
|Mouse VCL ORF mammalian expression plasmid, C-HA tag||MG50773-CY|
|Mouse VCL Gene cDNA clone plasmid||MG50773-G|
|Mouse VCL ORF mammalian expression plasmid, N-Flag tag||MG50773-NF|
|Mouse VCL ORF mammalian expression plasmid, N-His tag||MG50773-NH|
|Mouse VCL ORF mammalian expression plasmid, N-Myc tag||MG50773-NM|
|Mouse VCL ORF mammalian expression plasmid, N-HA tag||MG50773-NY|
|Mouse VCL natural ORF mammalian expression plasmid||MG50773-UT|
|Learn more about expression Vectors|
Vinculin (VCL) is a cytoskeletal protein that is closely related to both cell-matrix interactions and cell-cell junctions. VCL is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment. This protein has multi-ligand properties and has been found to interact with a number of microfilament associated proteins, such as talin, a-actinin, and paxillin, which reportedly bind to either the head or tail domains of vinculin.