|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse SELPLG ORF mammalian expression plasmid, C-GFPSpark tag||MG50770-ACG|
|Mouse SELPLG ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50770-ACR|
|Mouse SELPLG ORF mammalian expression plasmid, C-Flag tag||MG50770-CF|
|Mouse SELPLG ORF mammalian expression plasmid, C-His tag||MG50770-CH|
|Mouse SELPLG ORF mammalian expression plasmid, C-Myc tag||MG50770-CM|
|Mouse SELPLG ORF mammalian expression plasmid, C-HA tag||MG50770-CY|
|Mouse SELPLG Gene cDNA clone plasmid||MG50770-G|
|Mouse SELPLG ORF mammalian expression plasmid, N-Flag tag||MG50770-NF|
|Mouse SELPLG ORF mammalian expression plasmid, N-His tag||MG50770-NH|
|Mouse SELPLG ORF mammalian expression plasmid, N-Myc tag||MG50770-NM|
|Mouse SELPLG ORF mammalian expression plasmid, N-HA tag||MG50770-NY|
|Mouse SELPLG natural ORF mammalian expression plasmid||MG50770-UT|
|Learn more about expression Vectors|
P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinitycounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~120 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.
1. Sako, D. et al., 1993, Cell. 75: 1179-1186.
2. Wilkins, P. P. et al., 1995, J. Biol. Chem. 270: 22677-22680.
3. Frenette, P. S. et al., 2000, J. Exp. Med. 191: 1413-1422.
4. Vandendries, E.R .et al., 2004, Thromb. Haemost. 92: 459-466..
5. Pouyani, T. et al., 1995, Cell. 83: 333-343.