|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse NLGN1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50725-ACG|
|Mouse NLGN1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50725-ACR|
|Mouse NLGN1 ORF mammalian expression plasmid, C-Flag tag||MG50725-CF|
|Mouse NLGN1 ORF mammalian expression plasmid, C-His tag||MG50725-CH|
|Mouse NLGN1 ORF mammalian expression plasmid, C-Myc tag||MG50725-CM|
|Mouse NLGN1 ORF mammalian expression plasmid, C-HA tag||MG50725-CY|
|Mouse NLGN1 Gene cDNA clone plasmid||MG50725-M|
|Mouse NLGN1 ORF mammalian expression plasmid, N-Flag tag||MG50725-NF|
|Mouse NLGN1 ORF mammalian expression plasmid, N-His tag||MG50725-NH|
|Mouse NLGN1 ORF mammalian expression plasmid, N-Myc tag||MG50725-NM|
|Mouse NLGN1 ORF mammalian expression plasmid, N-HA tag||MG50725-NY|
|Mouse NLGN1 natural ORF mammalian expression plasmid||MG50725-UT|
|Learn more about expression Vectors|
Neuroligin 1 (NLGN1) belongs to the type-B carboxylesterase/lipase family, is a synaptic cell-adhesion molecule that is enriched in postsynaptic densities where it may recruit receptors, channels, and signal-transduction molecules to synaptic sites of cell adhesion. Neuroligins consist of five members (NLGN1, NLGN2, NLGN3, NLGN4 and NLGN4Y), which interact with beta-neurexins and this interaction is involved in the formation of functional synapses. The extracellular domain of functional Neuroligin 1 associates as a dimer when analyzed by sedimentation equilibrium. Neuroligin 1 has a unique N-linked glycosylation pattern in the neuroligin family, and glycosylation and its processing modify neuroligin activity. Neuroligin 1 is a potent trigger for the de novo formation of synaptic connections, and it has recently been suggested that it is required for the maturation of functionally competent excitatory synapses. The persistent expression of Neuroligin 1 is required for the maintenance of NMDAR-mediated synaptic transmission, which enables normal development of synaptic plasticity and long-term memory in the amygdala of adult animals.