|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CD96 ORF mammalian expression plasmid, C-GFPSpark tag||MG50758-ACG|
|Mouse CD96 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50758-ACR|
|Mouse CD96 ORF mammalian expression plasmid, C-Flag tag||MG50758-CF|
|Mouse CD96 ORF mammalian expression plasmid, C-His tag||MG50758-CH|
|Mouse CD96 ORF mammalian expression plasmid, C-Myc tag||MG50758-CM|
|Mouse CD96 ORF mammalian expression plasmid, C-HA tag||MG50758-CY|
|Mouse CD96 Gene cDNA clone plasmid||MG50758-G|
|Mouse CD96 ORF mammalian expression plasmid, N-Flag tag||MG50758-NF|
|Mouse CD96 ORF mammalian expression plasmid, N-His tag||MG50758-NH|
|Mouse CD96 ORF mammalian expression plasmid, N-Myc tag||MG50758-NM|
|Mouse CD96 ORF mammalian expression plasmid, N-HA tag||MG50758-NY|
|Mouse CD96 natural ORF mammalian expression plasmid||MG50758-UT|
|Learn more about expression Vectors|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. The CD155 ligand CD96 is a member of the Ig superfamily. It's a immunoglobulin-like protein tentatively allocated to the repertoire of human NK receptors. NK cells recognize poliovirus receptor (PVR), a nectins and nectin-like protein family member serve to mediate cell-cell adhesion, cell migration, with the presence of an additional receptor, CD96. CD96 promotes NK cell adhesion to target cells expressing PVR, stimulates cytotoxicity of activated NK cells, and mediates acquisition of PVR from target cells. The effect the cells with mutated CD96 protein lost adhesion and growth activities indicates that CD96 mutations may cause a form of the C syndrome by interfering with cell adhesion and growth.