|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CD84 ORF mammalian expression plasmid, C-GFPSpark tag||MG50757-ACG|
|Mouse CD84 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50757-ACR|
|Mouse CD84 ORF mammalian expression plasmid, C-Flag tag||MG50757-CF|
|Mouse CD84 ORF mammalian expression plasmid, C-His tag||MG50757-CH|
|Mouse CD84 ORF mammalian expression plasmid, C-Myc tag||MG50757-CM|
|Mouse CD84 ORF mammalian expression plasmid, C-HA tag||MG50757-CY|
|Mouse CD84 Gene cDNA clone plasmid||MG50757-G|
|Mouse CD84 ORF mammalian expression plasmid, N-Flag tag||MG50757-NF|
|Mouse CD84 ORF mammalian expression plasmid, N-His tag||MG50757-NH|
|Mouse CD84 ORF mammalian expression plasmid, N-Myc tag||MG50757-NM|
|Mouse CD84 ORF mammalian expression plasmid, N-HA tag||MG50757-NY|
|Mouse CD84 natural ORF mammalian expression plasmid||MG50757-UT|
|Learn more about expression Vectors|
The CD2 family receptors are type I transmembrane glycoproteins belonging to immunoglobulin (Ig) superfamily characterized by a membrane-proximal Ig constant 2 (C2) domain and a membrane-distal variable (V) domain that is responsible for ligand recognition. CD84, also known as LY9B and SLAMF5, is a homophilic member of the SLAM (signaling lymphocyte activation molecule) subfamily of the CD2 family. The SLAM family receptorsmediate signal transduction through the interaction of its ITSM (immunoreceptor tyrosine-based switch motifs) in the intracellular region and the SH2 domain of adaptor molecules SAP (SLAM-associated protein) and EAT-2 (EWS-activated transcript 2), and accordingly modulate both adaptive and innate immune responses. The CD84-CD84 interaction was independent of its cytoplasmic tail. Thus, CD84 is its own ligand and acts as a costimulatory molecule. CD84 is expressed on cells from almost all hematopoietic lineages and on CD34+ hematopoietic progenitor cells, suggesting that CD84 serves as a marker for committed hematopoietic progenitor cells.