|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse PARP1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50753-ACG|
|Mouse PARP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50753-ACR|
|Mouse PARP1 ORF mammalian expression plasmid, N-GFPSpark tag||MG50753-ANG|
|Mouse PARP1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50753-ANR|
|Mouse PARP1 ORF mammalian expression plasmid, C-Flag tag||MG50753-CF|
|Mouse PARP1 ORF mammalian expression plasmid, C-His tag||MG50753-CH|
|Mouse PARP1 ORF mammalian expression plasmid, C-Myc tag||MG50753-CM|
|Mouse PARP1 ORF mammalian expression plasmid, C-HA tag||MG50753-CY|
|Mouse PARP1 Gene cDNA clone plasmid||MG50753-G|
|Mouse PARP1 ORF mammalian expression plasmid, N-Flag tag||MG50753-NF|
|Mouse PARP1 ORF mammalian expression plasmid, N-His tag||MG50753-NH|
|Mouse PARP1 ORF mammalian expression plasmid, N-Myc tag||MG50753-NM|
|Mouse PARP1 ORF mammalian expression plasmid, N-HA tag||MG50753-NY|
|Mouse PARP1 natural ORF mammalian expression plasmid||MG50753-UT|
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Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrateed to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors is thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.