|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat TGM2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80519-ACG|
|Rat TGM2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80519-ACR|
|Rat TGM2 ORF mammalian expression plasmid, N-GFPSpark tag||RG80519-ANG|
|Rat TGM2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80519-ANR|
|Rat TGM2 ORF mammalian expression plasmid, C-Flag tag||RG80519-CF|
|Rat TGM2 ORF mammalian expression plasmid, C-His tag||RG80519-CH|
|Rat TGM2 ORF mammalian expression plasmid, C-Myc tag||RG80519-CM|
|Rat TGM2 ORF mammalian expression plasmid, C-HA tag||RG80519-CY|
|Rat TGM2 ORF mammalian expression plasmid, N-Flag tag||RG80519-NF|
|Rat TGM2 ORF mammalian expression plasmid, N-His tag||RG80519-NH|
|Rat TGM2 ORF mammalian expression plasmid, N-Myc tag||RG80519-NM|
|Rat TGM2 ORF mammalian expression plasmid, N-HA tag||RG80519-NY|
|Rat TGM2 Gene cDNA clone plasmid||RG80519-U|
|Rat TGM2 natural ORF mammalian expression plasmid||RG80519-UT|
|Learn more about expression Vectors|
Protein-glutamine gamma-glutamyltransferase 2, also known as Tissue transglutaminase, Transglutaminase C, Transglutaminase-2, and TGM2, is a member of the transglutaminase superfamily. TGM2 plays a role in cell growth and survival through the anti-apoptosis signaling pathway. It is a calcium-dependent acyltransferase which also undergoes a GTP-binding/GTPase cycle even though it lacks any obvious sequence similarity with canonical GTP-binding (G) proteins. TGM2 is a multi-functional protein which catalyzes transamidation reactions or acts as a G-protein in intracellular signalling. As an enzyme which is responsible for the majority of transglutaminase (TG) activity in the brain, TGM2 is likely to play a modulatory role in nervous system development and has regulatory effect on neuronal cell death as well. Most importantly, numerous studies have presented data demonstrating that dysregulation of TGM2 may contribute to the pathogenesis of many neurodegenerative disorders, including Huntington's disease, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis as well as nervous system injuries.