|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CD69 ORF mammalian expression plasmid, C-GFPSpark tag||MG50731-ACG|
|Mouse CD69 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50731-ACR|
|Mouse CD69 ORF mammalian expression plasmid, C-Flag tag||MG50731-CF|
|Mouse CD69 ORF mammalian expression plasmid, C-His tag||MG50731-CH|
|Mouse CD69 ORF mammalian expression plasmid, C-Myc tag||MG50731-CM|
|Mouse CD69 ORF mammalian expression plasmid, C-HA tag||MG50731-CY|
|Mouse CD69 Gene cDNA clone plasmid||MG50731-G|
|Mouse CD69 ORF mammalian expression plasmid, N-Flag tag||MG50731-NF|
|Mouse CD69 ORF mammalian expression plasmid, N-His tag||MG50731-NH|
|Mouse CD69 ORF mammalian expression plasmid, N-Myc tag||MG50731-NM|
|Mouse CD69 ORF mammalian expression plasmid, N-HA tag||MG50731-NY|
|Mouse CD69 natural ORF mammalian expression plasmid||MG50731-UT|
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Early activation antigen CD69, also known as activation inducer molecule (AIM), is a single-pass type II membrane protein. Recently, cDNA clones encoding human and mouse CD69 were isolated and showed CD69 to be a member of the C-type lectin superfamily. It is one of the earliest cell surface antigens expressed by T cells following activation. Once expressed, CD69 acts as a costimulatory molecule for T cell activation and proliferation. In addition to mature T cells, CD69 is inducibly expressed by immature thymocytes, B cells, natural killer (NK) cells, monocytes, neutrophils and eosinophils, and is constitutively expressed by mature thymocytes and platelets. CD69 is involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. The structure, chromosomal localization, expression and function of CD69 suggest that it is likely a pleiotropic immune regulator , potentially important in the activation and differentiation of a wide variety of hematopoietic cells. This membrane molecule transiently expresses on activated lymphocytes, and its selective expression in inflammatory infiltrates suggests that it plays a role in the pathogenesis of inflammatory diseases. CD69 plays a crucial role in the pathogenesis of allergen-induced eosinophilic airway inflammation and hyperresponsiveness and that CD69 could be a possible therapeutic target for asthmatic patients.