|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse Smad2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50727-ACG|
|Mouse Smad2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50727-ACR|
|Mouse Smad2 ORF mammalian expression plasmid, N-GFPSpark tag||MG50727-ANG|
|Mouse Smad2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50727-ANR|
|Mouse Smad2 ORF mammalian expression plasmid, C-Flag tag||MG50727-CF|
|Mouse Smad2 ORF mammalian expression plasmid, C-His tag||MG50727-CH|
|Mouse Smad2 ORF mammalian expression plasmid, C-Myc tag||MG50727-CM|
|Mouse Smad2 ORF mammalian expression plasmid, C-HA tag||MG50727-CY|
|Mouse Smad2 Gene cDNA clone plasmid||MG50727-G|
|Mouse Smad2 ORF mammalian expression plasmid, N-Flag tag||MG50727-NF|
|Mouse Smad2 ORF mammalian expression plasmid, N-His tag||MG50727-NH|
|Mouse Smad2 ORF mammalian expression plasmid, N-Myc tag||MG50727-NM|
|Mouse Smad2 ORF mammalian expression plasmid, N-HA tag||MG50727-NY|
|Mouse Smad2 natural ORF mammalian expression plasmid||MG50727-UT|
|Learn more about expression Vectors|
SMAD2 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD2 mediates the signal of the TGF-beta, and therefore regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. SMAD2 is the downstream signal transducers of TGF-beta-1 in human dental pulp cells. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. Phosphorylated SMAD2 is able to form a complex with SMAD4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression.