|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse SDC4 ORF mammalian expression plasmid, C-GFPSpark tag||MG50726-ACG|
|Mouse SDC4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50726-ACR|
|Mouse SDC4 ORF mammalian expression plasmid, C-Flag tag||MG50726-CF|
|Mouse SDC4 ORF mammalian expression plasmid, C-His tag||MG50726-CH|
|Mouse SDC4 ORF mammalian expression plasmid, C-Myc tag||MG50726-CM|
|Mouse SDC4 ORF mammalian expression plasmid, C-HA tag||MG50726-CY|
|Mouse SDC4 Gene cDNA clone plasmid||MG50726-G|
|Mouse SDC4 ORF mammalian expression plasmid, N-Flag tag||MG50726-NF|
|Mouse SDC4 ORF mammalian expression plasmid, N-His tag||MG50726-NH|
|Mouse SDC4 ORF mammalian expression plasmid, N-Myc tag||MG50726-NM|
|Mouse SDC4 ORF mammalian expression plasmid, N-HA tag||MG50726-NY|
|Mouse SDC4 natural ORF mammalian expression plasmid||MG50726-UT|
|Learn more about expression Vectors|
SDC4 (Syndecan-4), also known as Syn4, is a transmembrane heparan sulfate proteoglycan that co-operates with integrins during cell-matrix interactions for the assembly of focal adhesions and actin stress fibers and in the phosphorylation of focal adhesion kinase (FAK) on Tyr397. Syndecan-4 plays roles in the formation of focal adhesions and stress fibers. The cytoplasmic domain of syndecan-4 interacts with a number of signalling and structural proteins, and both extracellular and cytoplasmic domains are necessary for regulated activation of associated transmembrane receptors. Syndecan-4/SDC4 is a heparan sulfate proteoglycan and works as a coreceptor for various growth factors. SDC4 deficiency limits neointimal formation after vascular injury by regulating vascular smooth muscle cells (VSMCs) proliferation and vascular progenitor cells (VPCs) mobilization. Therefore, SDC4 may be a novel therapeutic target for preventing arterial restenosis after angioplasty.