|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CD59A ORF mammalian expression plasmid, C-GFPSpark tag||MG50724-ACG|
|Mouse CD59A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50724-ACR|
|Mouse CD59A ORF mammalian expression plasmid, C-Flag tag||MG50724-CF|
|Mouse CD59A ORF mammalian expression plasmid, C-His tag||MG50724-CH|
|Mouse CD59A ORF mammalian expression plasmid, C-Myc tag||MG50724-CM|
|Mouse CD59A ORF mammalian expression plasmid, C-HA tag||MG50724-CY|
|Mouse CD59A Gene cDNA clone plasmid||MG50724-G|
|Mouse CD59A ORF mammalian expression plasmid, N-Flag tag||MG50724-NF|
|Mouse CD59A ORF mammalian expression plasmid, N-His tag||MG50724-NH|
|Mouse CD59A ORF mammalian expression plasmid, N-Myc tag||MG50724-NM|
|Mouse CD59A ORF mammalian expression plasmid, N-HA tag||MG50724-NY|
|Mouse CD59A natural ORF mammalian expression plasmid||MG50724-UT|
|Learn more about expression Vectors|
Protectin, a complement regulatory protein, also known as CD59, or MIRL (membrane inhibitor of reactive lysis) is a small protein that inhibits the complement membrane attack complex by binding C5b678 and preventing C9 from binding and polymerizing. The amino-terminal 25 amino acids represented a typical signal peptide sequence and the carboxy-terminal hydrophobic amino acids were characteristic for phosphatidylinositol-anchored proteins.It was found that the CD59/Protectin antigen is a small protein sometimes associated with larger components (45 and 80 kD) in urine. CD59/Protectin antigen was released from the surface of transfected COS cells by phosphatidylinositol-specific phospholipase C, demonstrating that it is attached to the cell membrane by means of a glycolipid anchor; it is therefore likely to be absent from the surface of affected erythrocytes in the disease paroxysmal nocturnal hemoglobinuria.