|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human IFI30 ORF mammalian expression plasmid, C-GFPSpark tag||HG16347-ACG|
|Human IFI30 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG16347-ACR|
|Human IFI30 ORF mammalian expression plasmid, C-Flag tag||HG16347-CF|
|Human IFI30 ORF mammalian expression plasmid, C-His tag||HG16347-CH|
|Human IFI30 ORF mammalian expression plasmid, C-Myc tag||HG16347-CM|
|Human IFI30 ORF mammalian expression plasmid, C-HA tag||HG16347-CY|
|Human IFI30 Gene cDNA clone plasmid||HG16347-G|
|Human IFI30 ORF mammalian expression plasmid, N-Flag tag||HG16347-NF|
|Human IFI30 ORF mammalian expression plasmid, N-His tag||HG16347-NH|
|Human IFI30 ORF mammalian expression plasmid, N-Myc tag||HG16347-NM|
|Human IFI30 ORF mammalian expression plasmid, N-HA tag||HG16347-NY|
|Human IFI30 natural ORF mammalian expression plasmid||HG16347-UT|
|Learn more about expression Vectors|
IFI30 belongs to the GILT family. This family includes the two characterised human gamma-interferon-inducible lysosomal thiol reductase (GILT) sequences: P13284 and Q9UL08. It also contains several other eukaryotic putative proteins with similarity to GILT. The aligned region contains three conserved cysteine residues. In addition, the two GILT sequences possess a C-X(2)-C motif that is shared by some of the other sequences in the family. This motif is thought to be associated with disulphide bond reduction. IFI30 is a lysosomal thiol reductase that can reduce protein disulfide bonds. It facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. It also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation. IFI30 may facilitate the complete unfolding of proteins destined for lysosomal degradation and plays an important role in antigen processing.