|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat SERPINF1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80481-ACG|
|Rat SERPINF1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80481-ACR|
|Rat SERPINF1 ORF mammalian expression plasmid, C-Flag tag||RG80481-CF|
|Rat SERPINF1 ORF mammalian expression plasmid, C-His tag||RG80481-CH|
|Rat SERPINF1 ORF mammalian expression plasmid, C-Myc tag||RG80481-CM|
|Rat SERPINF1 ORF mammalian expression plasmid, C-HA tag||RG80481-CY|
|Rat SERPINF1 ORF mammalian expression plasmid, N-Flag tag||RG80481-NF|
|Rat SERPINF1 ORF mammalian expression plasmid, N-His tag||RG80481-NH|
|Rat SERPINF1 ORF mammalian expression plasmid, N-Myc tag||RG80481-NM|
|Rat SERPINF1 ORF mammalian expression plasmid, N-HA tag||RG80481-NY|
|Rat SERPINF1 Gene cDNA clone plasmid||RG80481-U|
|Rat SERPINF1 natural ORF mammalian expression plasmid||RG80481-UT|
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Pigment epithelium-derived factor, also known as PEDF, Serpin F1, and SERPINF1, is a multiple functional protein which has both anti-angiogenic activity and neurotrophic activity at the same time. PEDF is a secreted glycoprotein that belongs to the noninhibitory serpin. It has an alpha/beta core serine-protease inhibitor domain, three major beta-sheets, and ten alpha-helices. PEDF does not inhibit either serine or cysteine proteinases. PEDF exerts diverse physiological activities including anti-angiogenesis, anti-vasopermeability, anti-tumor, and neurotrophic activities. PEDF acts via multiple high affinity ligands and cell receptors. It has been described as a natural angiogenesis inhibitor with neurotrophic and immune-modulation properties. PEDF induces macrophages apoptosis and necrosis through the activation of peroxisome proliferator-activated receptor-gamma by which PEDF could modulate inflammatory reactions in septic shock. It balances angiogenesis in the eye and blocks tumor progression.