|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human PKM2 ORF mammalian expression plasmid, C-GFPSpark tag||HG11430-ACG|
|Human PKM2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11430-ACR|
|Human PKM2 ORF mammalian expression plasmid, N-GFPSpark tag||HG11430-ANG|
|Human PKM2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11430-ANR|
|Human PKM2 ORF mammalian expression plasmid, C-Flag tag||HG11430-CF|
|Human PKM2 ORF mammalian expression plasmid, C-His tag||HG11430-CH|
|Human PKM2 ORF mammalian expression plasmid, C-Myc tag||HG11430-CM|
|Human PKM2 ORF mammalian expression plasmid, C-HA tag||HG11430-CY|
|Human PKM2 Gene cDNA clone plasmid||HG11430-M|
|Human PKM2 ORF mammalian expression plasmid, N-Flag tag||HG11430-NF|
|Human PKM2 ORF mammalian expression plasmid, N-His tag||HG11430-NH|
|Human PKM2 ORF mammalian expression plasmid, N-Myc tag||HG11430-NM|
|Human PKM2 ORF mammalian expression plasmid, N-HA tag||HG11430-NY|
|Human PKM2 natural ORF mammalian expression plasmid||HG11430-UT|
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Pyruvate kinase isozymes M2 also known as pyruvate kinase muscle isozyme 2 (PKM2), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone-binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3), is an isoenzyme of the glycolytic enzyme pyruvate kinase. Pyruvate kinase isozymes M2 / PKM2 is a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. PKM2 has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. PKM2 functions as a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. PKM2 may stimulates POU5F1-mediated transcriptional activation. This protein Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms of PKM2 contributes to the control of glycolysis and is important for tumor cell proliferation and survival.