|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CD33 ORF mammalian expression plasmid, C-GFPSpark tag||MG50712-ACG|
|Mouse CD33 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50712-ACR|
|Mouse CD33 ORF mammalian expression plasmid, C-Flag tag||MG50712-CF|
|Mouse CD33 ORF mammalian expression plasmid, C-His tag||MG50712-CH|
|Mouse CD33 ORF mammalian expression plasmid, C-Myc tag||MG50712-CM|
|Mouse CD33 ORF mammalian expression plasmid, C-HA tag||MG50712-CY|
|Mouse CD33 Gene cDNA clone plasmid||MG50712-G|
|Mouse CD33 ORF mammalian expression plasmid, N-Flag tag||MG50712-NF|
|Mouse CD33 ORF mammalian expression plasmid, N-His tag||MG50712-NH|
|Mouse CD33 ORF mammalian expression plasmid, N-Myc tag||MG50712-NM|
|Mouse CD33 ORF mammalian expression plasmid, N-HA tag||MG50712-NY|
|Mouse CD33 natural ORF mammalian expression plasmid||MG50712-UT|
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Myeloid cell surface antigen CD33 also known as Sialic acid binding Ig-like lectin 3, CD33 antigen or Siglec-3, is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. This Single-pass type I membrane protein contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain. CD33 /Siglec-3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. CD33 /Siglec-3 preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33/Siglec-3 induces apoptosis in acute myeloid leukemia (in vitro). CD33/Siglec-3 can function as a sialic acid-dependent cell adhesion molecule and that binding can be modulated by endogenous sialoglycoconjugates when CD33 is expressed in a plasma membrane.