|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat PDCD1LG2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80454-ACG|
|Rat PDCD1LG2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80454-ACR|
|Rat PDCD1LG2 ORF mammalian expression plasmid, C-Flag tag||RG80454-CF|
|Rat PDCD1LG2 ORF mammalian expression plasmid, C-His tag||RG80454-CH|
|Rat PDCD1LG2 ORF mammalian expression plasmid, C-Myc tag||RG80454-CM|
|Rat PDCD1LG2 ORF mammalian expression plasmid, C-HA tag||RG80454-CY|
|Rat PDCD1LG2 Gene cDNA clone plasmid||RG80454-G|
|Rat PDCD1LG2 ORF mammalian expression plasmid, N-Flag tag||RG80454-NF|
|Rat PDCD1LG2 ORF mammalian expression plasmid, N-His tag||RG80454-NH|
|Rat PDCD1LG2 ORF mammalian expression plasmid, N-Myc tag||RG80454-NM|
|Rat PDCD1LG2 ORF mammalian expression plasmid, N-HA tag||RG80454-NY|
|Rat PDCD1LG2 natural ORF mammalian expression plasmid||RG80454-UT|
|Learn more about expression Vectors|
Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.