|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat SELP ORF mammalian expression plasmid, C-GFPSpark tag||RG80451-ACG|
|Rat SELP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80451-ACR|
|Rat SELP ORF mammalian expression plasmid, C-Flag tag||RG80451-CF|
|Rat SELP ORF mammalian expression plasmid, C-His tag||RG80451-CH|
|Rat SELP ORF mammalian expression plasmid, C-Myc tag||RG80451-CM|
|Rat SELP ORF mammalian expression plasmid, C-HA tag||RG80451-CY|
|Rat SELP Gene cDNA clone plasmid||RG80451-G|
|Rat SELP ORF mammalian expression plasmid, N-Flag tag||RG80451-NF|
|Rat SELP ORF mammalian expression plasmid, N-His tag||RG80451-NH|
|Rat SELP ORF mammalian expression plasmid, N-Myc tag||RG80451-NM|
|Rat SELP ORF mammalian expression plasmid, N-HA tag||RG80451-NY|
|Rat SELP natural ORF mammalian expression plasmid||RG80451-UT|
|Learn more about expression Vectors|
P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interacton of activated endothelial cells or platelets with leukocytes.