|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat PDGFRA ORF mammalian expression plasmid, C-GFPSpark tag||RG80433-ACG|
|Rat PDGFRA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80433-ACR|
|Rat PDGFRA ORF mammalian expression plasmid, C-Flag tag||RG80433-CF|
|Rat PDGFRA ORF mammalian expression plasmid, C-His tag||RG80433-CH|
|Rat PDGFRA ORF mammalian expression plasmid, C-Myc tag||RG80433-CM|
|Rat PDGFRA ORF mammalian expression plasmid, C-HA tag||RG80433-CY|
|Rat PDGFRA Gene cDNA clone plasmid||RG80433-G|
|Rat PDGFRA ORF mammalian expression plasmid, N-Flag tag||RG80433-NF|
|Rat PDGFRA ORF mammalian expression plasmid, N-His tag||RG80433-NH|
|Rat PDGFRA ORF mammalian expression plasmid, N-Myc tag||RG80433-NM|
|Rat PDGFRA ORF mammalian expression plasmid, N-HA tag||RG80433-NY|
|Rat PDGFRA natural ORF mammalian expression plasmid||RG80433-UT|
|Learn more about expression Vectors|
PDGFRA, also known as CD140a, together with the structurally homolog protein PDGFRB (CD140b), are cell surface receptors for members of the platelet-derived growth factor family. They are members of the class III subfamily of receptor tyrosine kinase (RTKs) with the similar structure characteristics of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGFRA is expressed in oligodendrocyte progenitor cells and mesothelial cell, and binds all three ligand isoforms PDGF-AA, PDGF-BB and PDGF-AB with high affinity, whereas PDGFRB dose not bind PDGF-AA. PDGFRA plays an essential role in regulating proliferation, chemotaxis and migration of mesangial cells. Recent studies have indicated that PDGFRA acts as a critical mediator of signaling in testis organogenesis and Leydig cell differentiation, and in addition, particularly important for kidney development. Additionally, PDGFRA is involved in tumor angiogenesis and maintenance of the tumor microenvironment and has been implicated in development and metastasis of Hepatocellular carcinoma (HCC). PDGFRA may represent a potential therapeutic target in thymic tumours. PDGFRA gene amplification rather than gene mutation may be the underlying genetic mechanism driving PDGFRA overexpression in a portion of gliomas.