|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat SPN ORF mammalian expression plasmid, C-GFPSpark tag||RG80432-ACG|
|Rat SPN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80432-ACR|
|Rat SPN ORF mammalian expression plasmid, C-Flag tag||RG80432-CF|
|Rat SPN ORF mammalian expression plasmid, C-His tag||RG80432-CH|
|Rat SPN ORF mammalian expression plasmid, C-Myc tag||RG80432-CM|
|Rat SPN ORF mammalian expression plasmid, C-HA tag||RG80432-CY|
|Rat SPN Gene cDNA clone plasmid||RG80432-G|
|Rat SPN ORF mammalian expression plasmid, N-Flag tag||RG80432-NF|
|Rat SPN ORF mammalian expression plasmid, N-His tag||RG80432-NH|
|Rat SPN ORF mammalian expression plasmid, N-Myc tag||RG80432-NM|
|Rat SPN ORF mammalian expression plasmid, N-HA tag||RG80432-NY|
|Rat SPN natural ORF mammalian expression plasmid||RG80432-UT|
|Learn more about expression Vectors|
CD43 is an abundantly expressed molecule on the T-cell surface that shows distinct localization to the migrating T-cell uropod and the distal pole complex (DPC) opposite the immunological synapse via association with the ezrin-radixin-moesin (ERM) family of actin regulatory proteins. CD43 has a 235-amino acid (aa) extracellular domain, a 23-aa transmembrane domain, and a 123-aa cytoplasmic domain, all encoded by a single exon. The intracytoplasmic region of the protein is necessary to transduce signals; it is rich in potentially phosphorylable threonines and serines but lacks tyrosine residues as well as catalytic activity. CD43 engagement on human peripheral blood T cells and monocytes leads to cell activation and proliferation through the generation of second messengers such as diacylglycerol and inositol phosphates, protein kinase C (PKC) activation and Ca2+ mobilization. In addition, CD43 ligation on human T cells induces the association of CD43 with Src family kinases, presumably through the interaction of their Src homology 3 domain with a proline-rich region of the CD43 intracytoplasmic tail. This molecule has been implicated in T cell activation, enhancing T cell response to allogeneic or mitogenic stimulation and CD43-specific signals have been reported to be sufficient to activate T cells in the absence of T cell receptor (TCR) engagement. In summary, CD43 regulates multiple T-cell functions, including T-cell activation, proliferation, apoptosis, and migration.