|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CNTN5 ORF mammalian expression plasmid, C-GFPSpark tag||RG80409-ACG|
|Rat CNTN5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80409-ACR|
|Rat CNTN5 ORF mammalian expression plasmid, C-Flag tag||RG80409-CF|
|Rat CNTN5 ORF mammalian expression plasmid, C-His tag||RG80409-CH|
|Rat CNTN5 ORF mammalian expression plasmid, C-Myc tag||RG80409-CM|
|Rat CNTN5 ORF mammalian expression plasmid, C-HA tag||RG80409-CY|
|Rat CNTN5 Gene cDNA clone plasmid||RG80409-G|
|Rat CNTN5 ORF mammalian expression plasmid, N-Flag tag||RG80409-NF|
|Rat CNTN5 ORF mammalian expression plasmid, N-His tag||RG80409-NH|
|Rat CNTN5 ORF mammalian expression plasmid, N-Myc tag||RG80409-NM|
|Rat CNTN5 ORF mammalian expression plasmid, N-HA tag||RG80409-NY|
|Rat CNTN5 natural ORF mammalian expression plasmid||RG80409-UT|
|Learn more about expression Vectors|
Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed mainly in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2(TAG-1), Contactin-3(BIG-1), BIG-2, Contactin-5(NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-5, also known as NB-2, is one of the neural recognition molecules in the contactin subgroup. Contactin-5 is expressed in brain and kidney and at very low level in placenta. In brain, Contactin-5 is highly expressed in the occipital lobe, amygdala, cerebral cortex, frontal lobe, thalamus and temporal lobe. Mice deficient in the Contactin-5 gene exhibit aberrant responses to acoustic stimuli. Contactin-5 may play a role in maturation of glutamatergic synapses in the brainstem during the final stages of auditory development. Contactin-5 gene may contribute to human neurological disorders.