|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse NOG / Noggin ORF mammalian expression plasmid, C-GFPSpark tag||MG50688-ACG|
|Mouse NOG / Noggin ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50688-ACR|
|Mouse NOG / Noggin ORF mammalian expression plasmid, C-Flag tag||MG50688-CF|
|Mouse NOG / Noggin ORF mammalian expression plasmid, C-His tag||MG50688-CH|
|Mouse NOG / Noggin ORF mammalian expression plasmid, C-Myc tag||MG50688-CM|
|Mouse NOG / Noggin ORF mammalian expression plasmid, C-HA tag||MG50688-CY|
|Mouse NOG / Noggin Gene cDNA clone plasmid||MG50688-G|
|Mouse NOG / Noggin natural ORF mammalian expression plasmid||MG50688-G-N|
|Mouse NOG / Noggin ORF mammalian expression plasmid, N-Flag tag||MG50688-NF|
|Mouse NOG / Noggin ORF mammalian expression plasmid, N-His tag||MG50688-NH|
|Mouse NOG / Noggin ORF mammalian expression plasmid, N-Myc tag||MG50688-NM|
|Mouse NOG / Noggin ORF mammalian expression plasmid, N-HA tag||MG50688-NY|
|Mouse NOG / Noggin natural ORF mammalian expression plasmid||MG50688-UT|
|Learn more about expression Vectors|
Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. It binds several BMPs with very high (picomolar) affinities, with a marked preference for BMP2 and BMP4 over BMP7. By binding tightly to BMPs, Noggin prevents BMPs from binding their receptors. Noggin binds the bone morphogenetic proteins (BMP) such as BMP-4 and BMP-7, and inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both type I and type II receptors. Interaction of BMP and its antagonist Noggin governs various developmental and cellular processes, including embryonic dorsal-ventral axis, induction of neural tissue, formation of joints in the skeletal system and neurogenesis in the adult brain. Noggin plays a key role in neural induction by inhibiting BMP4, along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development, joint formation, and neural tube fusion.