|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat NTRK1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80404-ACG|
|Rat NTRK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80404-ACR|
|Rat NTRK1 ORF mammalian expression plasmid, C-Flag tag||RG80404-CF|
|Rat NTRK1 ORF mammalian expression plasmid, C-His tag||RG80404-CH|
|Rat NTRK1 ORF mammalian expression plasmid, C-Myc tag||RG80404-CM|
|Rat NTRK1 ORF mammalian expression plasmid, C-HA tag||RG80404-CY|
|Rat NTRK1 Gene cDNA clone plasmid||RG80404-G|
|Rat NTRK1 ORF mammalian expression plasmid, N-Flag tag||RG80404-NF|
|Rat NTRK1 ORF mammalian expression plasmid, N-His tag||RG80404-NH|
|Rat NTRK1 ORF mammalian expression plasmid, N-Myc tag||RG80404-NM|
|Rat NTRK1 ORF mammalian expression plasmid, N-HA tag||RG80404-NY|
|Rat NTRK1 natural ORF mammalian expression plasmid||RG80404-UT|
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TRKA is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. It is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed. Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors. The presence of NTRK1 leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in TRKA gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. It was originally identified as an oncogene as it is commonly mutated in cancers, particularly colon and thyroid carcinomas. TRKA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. NTRK1 has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. It also activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis and thyroid papillary carcinoma.