|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse EIF2C2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50683-ACG|
|Mouse EIF2C2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50683-ACR|
|Mouse EIF2C2 ORF mammalian expression plasmid, N-GFPSpark tag||MG50683-ANG|
|Mouse EIF2C2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50683-ANR|
|Mouse EIF2C2 ORF mammalian expression plasmid, C-Flag tag||MG50683-CF|
|Mouse EIF2C2 ORF mammalian expression plasmid, C-His tag||MG50683-CH|
|Mouse EIF2C2 ORF mammalian expression plasmid, C-Myc tag||MG50683-CM|
|Mouse EIF2C2 ORF mammalian expression plasmid, C-HA tag||MG50683-CY|
|Mouse EIF2C2 Gene cDNA clone plasmid||MG50683-M|
|Mouse EIF2C2 ORF mammalian expression plasmid, N-Flag tag||MG50683-NF|
|Mouse EIF2C2 ORF mammalian expression plasmid, N-His tag||MG50683-NH|
|Mouse EIF2C2 ORF mammalian expression plasmid, N-Myc tag||MG50683-NM|
|Mouse EIF2C2 ORF mammalian expression plasmid, N-HA tag||MG50683-NY|
|Mouse EIF2C2 natural ORF mammalian expression plasmid||MG50683-UT|
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Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.