|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat OPCML ORF mammalian expression plasmid, C-GFPSpark tag||RG80397-ACG|
|Rat OPCML ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80397-ACR|
|Rat OPCML ORF mammalian expression plasmid, C-Flag tag||RG80397-CF|
|Rat OPCML ORF mammalian expression plasmid, C-His tag||RG80397-CH|
|Rat OPCML ORF mammalian expression plasmid, C-Myc tag||RG80397-CM|
|Rat OPCML ORF mammalian expression plasmid, C-HA tag||RG80397-CY|
|Rat OPCML Gene cDNA clone plasmid||RG80397-G|
|Rat OPCML ORF mammalian expression plasmid, N-Flag tag||RG80397-NF|
|Rat OPCML ORF mammalian expression plasmid, N-His tag||RG80397-NH|
|Rat OPCML ORF mammalian expression plasmid, N-Myc tag||RG80397-NM|
|Rat OPCML ORF mammalian expression plasmid, N-HA tag||RG80397-NY|
|Rat OPCML natural ORF mammalian expression plasmid||RG80397-UT|
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Opioid-binding Cell Adhesion Molecule (OBCAM), also known as OPCML, is a GPI-anchored cell adhesion molecule in the plasma membrane. This neuron-specific protein, consists of three immunoglobulin (Ig)-like domains anchored to the membrane through a glycosylphosphatidylinositol (GPI)-tail. OPCML also belongs to the member of the IgLON family, a subgroup of the immunoglobulin superfamily, consisting of three members, LAMP, OBCAM, and Neurotrimin. These molecules interact homophilically and heterophilically within the family, and OBCAM acts only as heterodimers with LAMP or Neurotrimin and possibly inhibits neurite outgrowth from cerebellar granule cells. OBCAM has been presumed to play a role as a cell adhesion/recognition molecule. Furthermore, the OPCML protein defects may play an important role in the carcinogenesis of cervical or ovarian cancers, and this gene is regarded as a candidate TSG (tumor suppressor gene).