|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse ADIPOQ ORF mammalian expression plasmid, C-GFPSpark tag||MG50636-ACG|
|Mouse ADIPOQ ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50636-ACR|
|Mouse ADIPOQ ORF mammalian expression plasmid, C-Flag tag||MG50636-CF|
|Mouse ADIPOQ ORF mammalian expression plasmid, C-His tag||MG50636-CH|
|Mouse ADIPOQ ORF mammalian expression plasmid, C-Myc tag||MG50636-CM|
|Mouse ADIPOQ ORF mammalian expression plasmid, C-HA tag||MG50636-CY|
|Mouse ADIPOQ Gene cDNA clone plasmid||MG50636-M|
|Mouse ADIPOQ ORF mammalian expression plasmid, N-Flag tag||MG50636-NF|
|Mouse ADIPOQ ORF mammalian expression plasmid, N-His tag||MG50636-NH|
|Mouse ADIPOQ ORF mammalian expression plasmid, N-Myc tag||MG50636-NM|
|Mouse ADIPOQ ORF mammalian expression plasmid, N-HA tag||MG50636-NY|
|Mouse ADIPOQ natural ORF mammalian expression plasmid||MG50636-UT|
|Learn more about expression Vectors|
Adiponectin (ADIPOQ), or 30 kDa adipocyte complement-related protein (Acrp30) is a protein secreted by adipose tissue, which acts to reduce insulin resistance and atherogenic damage, but it also exerts actions in other tissues. Adiponectin mediates its actions in the periphery mainly via two receptors, AdipoR1 and AdipoR2. Adiponectin influences gonadotropin release, normal pregnancy, and assisted reproduction outcomes. Adiponectin, a beneficial adipokine, represents a major link between obesity and reproduction. Higher levels of adiponectin are associated with improved menstrual function and better outcomes in assisted reproductive cycles. Unlike other adipocytokines produced by adipose tissue, adiponectin appears to have anti-inflammatory, anti-diabetic, and anti-atherogenic properties. Several clinical studies demonstrate the inverse relationship between plasma adiponectin levels and several inflammatory markers including C-reactive protein. Adiponectin attenuates inflammatory responses to multiple stimuli by modulating signaling pathways in a variety of cell types. The anti-inflammatory properties of adiponectin may be a major component of its beneficial effects on cardiovascular and metabolic disorders including atherosclerosis and insulin resistance. Additionally, it is important factor in chronic liver diseases and chronic kidney diseases. Some cancer cell types express adiponectin receptors. Thus Adiponectin may act on tumour cells directly by binding and activating adiponectin receptors and downstream signalling pathways.