|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CD300A ORF mammalian expression plasmid, C-GFPSpark tag||MG50675-ACG|
|Mouse CD300A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50675-ACR|
|Mouse CD300A ORF mammalian expression plasmid, C-Flag tag||MG50675-CF|
|Mouse CD300A ORF mammalian expression plasmid, C-His tag||MG50675-CH|
|Mouse CD300A ORF mammalian expression plasmid, C-Myc tag||MG50675-CM|
|Mouse CD300A ORF mammalian expression plasmid, C-HA tag||MG50675-CY|
|Mouse CD300A Gene cDNA clone plasmid||MG50675-M|
|Mouse CD300A ORF mammalian expression plasmid, N-Flag tag||MG50675-NF|
|Mouse CD300A ORF mammalian expression plasmid, N-His tag||MG50675-NH|
|Mouse CD300A ORF mammalian expression plasmid, N-Myc tag||MG50675-NM|
|Mouse CD300A ORF mammalian expression plasmid, N-HA tag||MG50675-NY|
|Mouse CD300A natural ORF mammalian expression plasmid||MG50675-UT|
|Learn more about expression Vectors|
Mouse CMRF35-like molecule 8, also known as CD300 antigen-like family member A, CMRF35-H9, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, NK inhibitory receptor, CD300a and CMRF35H, is a single-pass type I membrane protein which belongs to the CD300 family. The CD300 family of myeloid immunoglobulin receptors includes activating ( CD300b, CD300e ) and inhibitory members ( CD300a, CD300f ), as well as molecules presenting a negative charge within their transmembrane domain ( CD300c, CD300d ). CD300A / IGSF12 is expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes.It contains one Ig-like V-type ( immunoglobulin-like ) domain. CD300A / IGSF12 is an inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. CD300c is a functional immune receptor able to deliver activating signals upon ligation in RBL-2H3 mast cells. CD300c signaling is partially mediated by a direct association with the immune receptor tyrosine-based activation motif-bearing adaptor FcεRγ. CD300a and CD300c play an important role in the cross-regulation of TNF-alpha and IFN-alpha secretion from plasmacytoid dendritic cells (pDCs).