|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CD276 ORF mammalian expression plasmid, C-GFPSpark tag||RG80380-ACG|
|Rat CD276 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80380-ACR|
|Rat CD276 ORF mammalian expression plasmid, C-Flag tag||RG80380-CF|
|Rat CD276 ORF mammalian expression plasmid, C-His tag||RG80380-CH|
|Rat CD276 ORF mammalian expression plasmid, C-Myc tag||RG80380-CM|
|Rat CD276 ORF mammalian expression plasmid, C-HA tag||RG80380-CY|
|Rat CD276 Gene cDNA clone plasmid||RG80380-G|
|Rat CD276 ORF mammalian expression plasmid, N-Flag tag||RG80380-NF|
|Rat CD276 ORF mammalian expression plasmid, N-His tag||RG80380-NH|
|Rat CD276 ORF mammalian expression plasmid, N-Myc tag||RG80380-NM|
|Rat CD276 ORF mammalian expression plasmid, N-HA tag||RG80380-NY|
|Rat CD276 natural ORF mammalian expression plasmid||RG80380-UT|
|Learn more about expression Vectors|
B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.