|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human FLRT1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11389-ACG|
|Human FLRT1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11389-ACR|
|Human FLRT1 ORF mammalian expression plasmid, C-Flag tag||HG11389-CF|
|Human FLRT1 ORF mammalian expression plasmid, C-His tag||HG11389-CH|
|Human FLRT1 ORF mammalian expression plasmid, C-Myc tag||HG11389-CM|
|Human FLRT1 ORF mammalian expression plasmid, C-HA tag||HG11389-CY|
|Human FLRT1 Gene cDNA clone plasmid||HG11389-M|
|Human FLRT1 ORF mammalian expression plasmid, Flag tag||HG11389-M-F|
|Human FLRT1 ORF mammalian expression plasmid, N-Flag tag||HG11389-NF|
|Human FLRT1 ORF mammalian expression plasmid, N-His tag||HG11389-NH|
|Human FLRT1 ORF mammalian expression plasmid, N-Myc tag||HG11389-NM|
|Human FLRT1 ORF mammalian expression plasmid, N-HA tag||HG11389-NY|
|Human FLRT1 natural ORF mammalian expression plasmid||HG11389-UT|
|Learn more about expression Vectors|
The three fibronectin leucine-rich repeat transmembrane (FLRT) proteins contain 10 leucine-rich repeats (LRR), a type III fibronectin (FN) domain, followed by the transmembrane region, and a short cytoplasmic tail. FLRT1 is expressed in kidney and brain, which is a target for tyrosine phosphorylation mediated by FGFR1 and implicate a non-receptor Src family kinase (SFK). All FLRTs can interact with FGFR1 and FLRTs can be induced by the activation of FGF signalling by FGF-2. The phosphorylation state of FLRT1, which is itself FGFR1 dependent, may play a critical role in the potentiation of FGFR1 signalling and may also depend on a SFK-dependent phosphorylation mechanism acting via the FGFR. This is consistent with an 'in vivo' role for FLRT1 regulation of FGF signalling via SFKs. Furthermore, the phosphorylation-dependent futile cycle mechanism controlling FGFR1 signalling is concurrently crucial for regulation of FLRT1-mediated neurite outgrowth. FLRT1, FLRT2 and FLRT3 are members of the fibronectin leucine rich transmembrane protein (FLRT) family. They may function in cell adhesion and/or receptor signalling. Their protein structures resemble small leucine-rich proteoglycans found in the extracellular matrix. FLRT3 shares 55% amino acid sequence identity with FLRT1.