|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse IFNGR2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50665-ACG|
|Mouse IFNGR2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50665-ACR|
|Mouse IFNGR2 ORF mammalian expression plasmid, C-Flag tag||MG50665-CF|
|Mouse IFNGR2 ORF mammalian expression plasmid, C-His tag||MG50665-CH|
|Mouse IFNGR2 ORF mammalian expression plasmid, C-Myc tag||MG50665-CM|
|Mouse IFNGR2 ORF mammalian expression plasmid, C-HA tag||MG50665-CY|
|Mouse IFNGR2 Gene cDNA clone plasmid||MG50665-M|
|Mouse IFNGR2 ORF mammalian expression plasmid, N-Flag tag||MG50665-NF|
|Mouse IFNGR2 ORF mammalian expression plasmid, N-His tag||MG50665-NH|
|Mouse IFNGR2 ORF mammalian expression plasmid, N-Myc tag||MG50665-NM|
|Mouse IFNGR2 ORF mammalian expression plasmid, N-HA tag||MG50665-NY|
|Mouse IFNGR2 natural ORF mammalian expression plasmid||MG50665-UT|
|Learn more about expression Vectors|
Interferon gamma receptor beta chain (IFNgammaR2), also known as IFNGR2, belongs to the type II cytokine receptor family, whose deficiency is a cause of autosomal recessive mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. This accessory factor is an integral part of the IFN-gamma signal transduction pathway and is likely to interact with GAF, JAK1, and/or JAK2. IFNGR2 is a component of the IFNgamma receptor complex along with the IFNgammaR alpha chain (IFNGR1), and is a new Bax suppressor. The C-terminal fragment (cytoplasmic domain) of IFNgammaR2 is expressed in human cancer cell lines of megakaryocytic cancer (DAMI), breast cancer (MDA-MD-468), and prostate cancer (PC3 cells). The Th1 cytokine IFNgamma, acting through its heterodimeric receptors, IFNgammaR1 and IFNgammaR2, in the induction/proliferation of Th1 cells, might suppress the Th2 responses that may underlie atopic asthma. IFNGR2 has always been seen as a key mechanism for shielding T lymphocytes from the antiproliferative effects of the IFNgamma-signal transducer and activator of transcription 1 (STAT1) pathway.