|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat SLAMF1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80349-ACG|
|Rat SLAMF1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80349-ACR|
|Rat SLAMF1 ORF mammalian expression plasmid, C-Flag tag||RG80349-CF|
|Rat SLAMF1 ORF mammalian expression plasmid, C-His tag||RG80349-CH|
|Rat SLAMF1 ORF mammalian expression plasmid, C-Myc tag||RG80349-CM|
|Rat SLAMF1 ORF mammalian expression plasmid, C-HA tag||RG80349-CY|
|Rat SLAMF1 Gene cDNA clone plasmid||RG80349-G|
|Rat SLAMF1 ORF mammalian expression plasmid, N-Flag tag||RG80349-NF|
|Rat SLAMF1 ORF mammalian expression plasmid, N-His tag||RG80349-NH|
|Rat SLAMF1 ORF mammalian expression plasmid, N-Myc tag||RG80349-NM|
|Rat SLAMF1 ORF mammalian expression plasmid, N-HA tag||RG80349-NY|
|Rat SLAMF1 natural ORF mammalian expression plasmid||RG80349-UT|
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CD150/signaling lymphocytic activation molecule (SLAM) is a cell surface sialylated phosphoglycoprotein and belongs to the CD2 subset of the Ig superfamily of type I transmembrane glycoproteins. The CD150 receptor is expressed on thymocytes, activated and memory T cells, B cells, platelets, natural killer T cells, and mature dendritic cells, and is also detected on tumor cells of Hodgkin's lymphoma (HL) and diffuse large B-cell lymphoma with an activated B cell phenotype. Additionally, it is the immune cell receptor for measles virus (MV). As a self-ligand, CD150 performs diverse immunologic functions including T/B-cell costimulation, induction of IFN-&gamma in Th1 T-cell clones, redirection of Th2 clones to a Th1 or Th0 phenotype, and inhibition of apoptosis in B cells. Furthermore, CD150 was shown to be the second receptor for measles virus in addition to CD46, and the distribution of SLAM on various cell lines is consistent with their susceptibility to clinical isolates of measles virus.