|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse FABP4 ORF mammalian expression plasmid, C-GFPSpark tag||MG50652-ACG|
|Mouse FABP4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50652-ACR|
|Mouse FABP4 ORF mammalian expression plasmid, N-GFPSpark tag||MG50652-ANG|
|Mouse FABP4 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50652-ANR|
|Mouse FABP4 ORF mammalian expression plasmid, C-Flag tag||MG50652-CF|
|Mouse FABP4 ORF mammalian expression plasmid, C-His tag||MG50652-CH|
|Mouse FABP4 ORF mammalian expression plasmid, C-Myc tag||MG50652-CM|
|Mouse FABP4 ORF mammalian expression plasmid, C-HA tag||MG50652-CY|
|Mouse FABP4 Gene cDNA clone plasmid||MG50652-G|
|Mouse FABP4 ORF mammalian expression plasmid, N-Flag tag||MG50652-NF|
|Mouse FABP4 ORF mammalian expression plasmid, N-His tag||MG50652-NH|
|Mouse FABP4 ORF mammalian expression plasmid, N-Myc tag||MG50652-NM|
|Mouse FABP4 ORF mammalian expression plasmid, N-HA tag||MG50652-NY|
|Mouse FABP4 natural ORF mammalian expression plasmid||MG50652-UT|
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Mouse fatty acid-binding protein, adipocyte, also known as Adipocyte-type fatty acid-binding protein, Fatty acid-binding protein 4, Adipocyte lipid-binding protein, and FABP4, is a cytoplasm protein which belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. In familial combined hyperlipidemia (FCHL), FABP4 correlated to body mass index (BMI), waist circumference and homeostasis model assessment (HOMA) index.FABP4 levels were associated with triglyceride-rich lipoproteins. In humans serum FABP4 levels correlate significantly with features of PCOS. It appears to be a determinant of atherogenic dyslipidemia. FABP4 pathway mediates the sebaceous gland hyperplasia in keratinocyte-specific Pten-null mice. FABP4 concentration significantly increased with an increasing of MS features and was strongly correlated with body-mass index, triglycerides, HDL-cholesterol concentrations and blood pressure. Patients in the highest quartile of FABP4 presented a six-fold increased odds ratio for MS and a three-fold increased odds for LD, adjusted by age, sex, body-mass index and the antiretroviral therapy. FABP4 is a strong plasma marker of metabolic disturbances in HIV-infected patients, and therefore, could serve to guide therapeutic intervention in this group of patients.