|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat THY1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80337-ACG|
|Rat THY1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80337-ACR|
|Rat THY1 ORF mammalian expression plasmid, C-Flag tag||RG80337-CF|
|Rat THY1 ORF mammalian expression plasmid, C-His tag||RG80337-CH|
|Rat THY1 ORF mammalian expression plasmid, C-Myc tag||RG80337-CM|
|Rat THY1 ORF mammalian expression plasmid, C-HA tag||RG80337-CY|
|Rat THY1 Gene cDNA clone plasmid||RG80337-G|
|Rat THY1 ORF mammalian expression plasmid, N-Flag tag||RG80337-NF|
|Rat THY1 ORF mammalian expression plasmid, N-His tag||RG80337-NH|
|Rat THY1 ORF mammalian expression plasmid, N-Myc tag||RG80337-NM|
|Rat THY1 ORF mammalian expression plasmid, N-HA tag||RG80337-NY|
|Rat THY1 natural ORF mammalian expression plasmid||RG80337-UT|
|Learn more about expression Vectors|
Mouse Thy-1 membrane glycoprotein, also known as Thy-1 antigen, CD90 and THY1, is a cell membrane protein which contains 1 Ig-like V-type (immunoglobulin-like) domain. It is a glycophosphatidylinositol-linked glycoprotein expressed on the surface of neurons, thymocytes, subsets of fibroblasts, endothelial cells, mesangial cells and some hematopoietic cells. It has been identified on a variety of stem cells and at varying levels in non-lymphoid tissues such as on fibroblasts, brain cells, and activated endothelial cells. Thy-1 is evolutionarily conserved, developmentally regulated, and often has dramatic effects on cell phenotype. Thy-1 is a 25-37 kDa glycosylphosphatidylinositol (GPI)-anchored protein involved in T cell activation, neurite outgrowth, apoptosis, tumor suppression, wound healing, and fibrosis. To mediate these diverse effects, Thy-1 participates in multiple signaling cascades. Thy-1 is an important regulator of cell-cell and cell-matrix interactions, with important roles in nerve regeneration, metastasis, inflammation, and fibrosis.