|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat NT5E ORF mammalian expression plasmid, C-GFPSpark tag||RG80335-ACG|
|Rat NT5E ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80335-ACR|
|Rat NT5E ORF mammalian expression plasmid, C-Flag tag||RG80335-CF|
|Rat NT5E ORF mammalian expression plasmid, C-His tag||RG80335-CH|
|Rat NT5E ORF mammalian expression plasmid, C-Myc tag||RG80335-CM|
|Rat NT5E ORF mammalian expression plasmid, C-HA tag||RG80335-CY|
|Rat NT5E Gene cDNA clone plasmid||RG80335-G|
|Rat NT5E ORF mammalian expression plasmid, N-Flag tag||RG80335-NF|
|Rat NT5E ORF mammalian expression plasmid, N-His tag||RG80335-NH|
|Rat NT5E ORF mammalian expression plasmid, N-Myc tag||RG80335-NM|
|Rat NT5E ORF mammalian expression plasmid, N-HA tag||RG80335-NY|
|Rat NT5E natural ORF mammalian expression plasmid||RG80335-UT|
|Learn more about expression Vectors|
5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the 5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.