|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat LILRA5 ORF mammalian expression plasmid, C-GFPSpark tag||RG80334-ACG|
|Rat LILRA5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80334-ACR|
|Rat LILRA5 ORF mammalian expression plasmid, C-Flag tag||RG80334-CF|
|Rat LILRA5 ORF mammalian expression plasmid, C-His tag||RG80334-CH|
|Rat LILRA5 ORF mammalian expression plasmid, C-Myc tag||RG80334-CM|
|Rat LILRA5 ORF mammalian expression plasmid, C-HA tag||RG80334-CY|
|Rat LILRA5 Gene cDNA clone plasmid||RG80334-G|
|Rat LILRA5 ORF mammalian expression plasmid, N-Flag tag||RG80334-NF|
|Rat LILRA5 ORF mammalian expression plasmid, N-His tag||RG80334-NH|
|Rat LILRA5 ORF mammalian expression plasmid, N-Myc tag||RG80334-NM|
|Rat LILRA5 ORF mammalian expression plasmid, N-HA tag||RG80334-NY|
|Rat LILRA5 natural ORF mammalian expression plasmid||RG80334-UT|
|Learn more about expression Vectors|
LILRA5 is a member of the leukocyte immunoglobulin-like receptor (LIR) family. LILR are a family of receptors possessing extracellular immunoglobulin domains. They are also known as CD85, ILTs and LIR, and can exert immunomodulatory effects on a wide range of immune cells. ILT-11 contains 2 Ig-like C2-type (immunoglobulin-like) domains. It can be detected n tissues of the hematopoietic system, including bone marrow, spleen, lymph node and peripheral leukocytes. Crosslink of ILT-11 on the surface of monocytes has been shown to induce calcium flux and secretion of several proinflammatory cytokines, which suggests the roles of this protein in triggering innate immune responses.